vietnam association of physiology
VIETNAMESE GINSENG EXTRACT ATTENUATES OXIDATIVE STRESS IN
COBALT CHLORIDE-SUBJECTED H9C2 CELLS
Ngo Thi Hai Yen1,2,#, Vu Thi Thu1,*,#
Objective: This study aimed to evaluate the protective effects of Vietnamese ginseng extract (VGE) on the formation of reactive oxygen species (ROS) in Cobalt chloride (CoCl2)-exposed H9C2 cells. Methods: H9C2 cells were subjected to CoCl₂-induced hypoxia-reoxygenation (HR) model with or without treatment of VGE. The hydroperoxide (H2O2) and superoxide (O2-) productions in H9C2 cells in the experimental groups were assessed by suitable fluorescence kits. Results: The obtained data showed that VGE at a dose of 31.25 µg/ml significantly reduced ROS levels in HR-subjected H9C2 cells. In the HR group, the H2O2 and O2- content in H9C2 cells were sharply increased to 156.81±7.82% and 160.10±2.07% (of 100% control), respectively. Interestingly, in the VGE-treated HR group, these indicators were significantly decreased to 119.67± 3.37% and 124.72 ± 3.21% (p <0.05), respectively. Conclusion: The results demonstrated that VGE effectively attenuated oxidative stress in cardiomyocytes under CoCl2-induced HR condition.
Key word: H9C2, reactive oxygen species, CoCl2.